Perform study design, isolation and applications of primary cell cultures, organoid culture, in vivo optogenetics, fluorescence and bioluminescence imaging in vivo and in vitro (IVIS Spectrum In Vivo Imaging System), endotoxin-free plasmid DNA purification, production and purification of adenoviral vectors, stereotaxic and retro-orbital (blood-brain permeable AAVs) injection, hydrodynamic tail vein injection of plasmid DNA, cell transfection in vitro.

Skills: Grant application, project management, study design, assays on primary cell cultures, mouse models of neuroinflammation, spectrofluorimetric registration of changes in membrane potential, intracellular pH, cytosolic Na+ and Ca2+ concentrations, FM1-43 destaining, endogenous glutamate release (glutamate dehydrogenase assay), fluorescence microscopy.

Experienced with Origin Pro, Zeiss LSM Image Browser, ImageJ, FlowJo, Adobe Photoshop, Microsoft Excel/Word/PowerPoint, Chem3D Ultra, BioDraw Ultra, Biorender. Surgery: craniotomy surgery on mice for mounting of a chronic cranial window, intracerebral AAV injection, brain perfusion fixation with cryosectioning.

Area of interest: Neurotransmission, neuroinflammation, strategies for neuroprotection, neurogenesis, regulation of secretion in synapse, synaptic vesicle recycling, SNARE-mediated membrane fusion, neurotransmitter turnover, ionotropic glutamate receptors, live cell imaging

Languages:

Ukrainian (native), Russian (fluent), English (C2)

Grant application, project coordination, study design, search for new targets in neuroinflammatory conditions, in vivo applications, mouse models of pathological conditions, publication of articles